How to reload init.el file without restart emacs

[lvyiwang . com]

emacs edition: 24.2
platform: window 7

I have tried these methods, but they all don't work well.

C-x C-e ;; current line
M-x eval-region ;; region
M-x eval-buffer ;; whole buffer
M-x load-file ~/.emacs.d/init.el

What was wrong:
My settings:
(global-set-key (kbd "C-<") 'mc/mark-previous-like-this)
I toggle this line with ;;
And I used those methods, but "C-<" still works.

So, what's wrong? Thanks!